Thermo Fisher Scientific Phospho-SMAD3 (Ser423, Ser425) Recombinant Rabbit Monoclonal Antibody (16H5L12)
Phospho-SMAD3 (Ser423, Ser425) 인산화 단백질을 검출하는 재조합 토끼 단클론 항체. Western blot 및 ICC/IF에 적합하며, 높은 특이성과 재현성을 제공. Protein A 정제, PBS 완충액에 0.09% sodium azide 포함. 단기 4°C, 장기 -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 16H5L12 |
| Immunogen | Peptide corresponding to Human SMAD3 (aa 418–425) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2633004 |
Product Specific Information
This antibody is predicted to react with Monkey, Sheep, Rat, and Pig.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone specific antibody DNA sequences from immunoreactive rabbits, allowing screening and selection of optimal clones for production. Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to rabbit immune repertoire
Target Information
SMAD3 is a member of the SMAD family of proteins, homologous to the Drosophila gene mothers against decapentaplegic (Mad) and the C. elegans gene Sma. SMAD proteins act as signal transducers and transcriptional modulators mediating multiple signaling pathways. SMAD3 functions as a transcriptional modulator activated by transforming growth factor-beta (TGF-β) and is implicated in carcinogenesis regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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