Thermo Fisher Scientific GFP Polyclonal Antibody, HRP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:2,000

View 18 publications 18 publications

Immunohistochemistry (IHC)

1:200-1:2,000

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-

View 3 publications 3 publications

ELISA (ELISA)

-

View 2 publications 2 publications

Immunoprecipitation (IP)

-

View 3 publications 3 publications

Product Specifications

Species Reactivity

Tag

Published species

Plant, Tag

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The GFP was isolated directly from the jellyfish Aequorea victoria.

Conjugate

HRP HRP HRP

Form

Lyophilized

Purification

Ion-exchange chromatography

Storage buffer

PBS, pH 7.4

Contains

0.02% thimerosal

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2534022

Product Specific Information

We offer the anti-GFP antibody HRP as purified rabbit IgG fraction and conjugated to horseradish peroxidase (HRP). At the time of preparation, the products are certified to be free of unconjugated dyes and are tested in a cytological experiment to ensure low nonspecific staining. This anti-GFP antibody HRP conjugate is suited for detection of native GFP, GFP variants, and most GFP-fusion proteins by western blot analysis and immunocytochemistry.

For initial experiments, we recommend trying dilutions ranging from 1:200 to 1:2,000 for immunocytochemical applications and western blot analysis. It is a good practice to centrifuge the protein conjugate solutions briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step eliminates any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.

Storage and reconstitution: when stored desiccated at or below -20°C, the lyophilized powder is stable for at least one year. Reconstitute using 200 µL PBS, pH 7.2, to yield a 1 mg/mL stock solution containing 0.02% thimerosal. After reconstitution, store product at 4°C.

For prolonged storage after reconstitution, add glycerol to a final concentration of 50% (v/v), aliquot, and store at or below -20°C. When stored properly, the solution is stable for approximately three months.

Target Information

The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used extensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.