Thermo Fisher Scientific GFP Polyclonal Antibody
GFP 단백질을 인식하는 Thermo Fisher Scientific의 폴리클로날 항체로, Western blot 및 ICC/IF 등 다양한 응용에 사용 가능. 정제된 액상 형태로 4°C 보관. 연구용으로 유전자 발현 및 단백질 위치 연구에 적합.
- 카탈로그번호
- A10262
- 판매단위
- pk
카탈로그
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 20 publications |
| Immunohistochemistry (IHC) | - | View 279 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 4 publications |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | - | View 2 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 20 publications |
| Immunohistochemistry - Free Floating (IHC (Free)) | - | View 4 publications |
| Immunocytochemistry (ICC/IF) | 1:100 | View 89 publications |
| Flow Cytometry (Flow) | - | View 3 publications |
| Immunoprecipitation (IP) | - | View 1 publication |
| Miscellaneous PubMed (Misc) | - | View 6 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Published Species | Tag |
| Host / Isotype | Chicken / IgY |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | The GFP was isolated directly from the jellyfish Aequorea victoria. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 5 mM sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2534023 |
Target Information
The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP is widely used as a reporter protein for gene expression in eukaryotic and prokaryotic organisms and as a protein tag in cell culture and multicellular organisms.
As a fusion tag, GFP can be used to localize proteins, study their movement, or research the dynamics of subcellular compartments. GFP technology has provided new insights into the physiological activities of living cells. GFP is a 27 kDa monomeric protein that autocatalytically forms a fluorescent pigment, absorbing blue light (395 nm) and emitting green light (508 nm) without additional cofactors.
GFP fluorescence is stable and species-independent, suitable for various applications such as monitoring gene expression, protein localization, protein-protein interactions (yeast two-hybrid system), and fluorescence energy transfer (FRET) experiments.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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