Ion Torrent™

Ion Library Equalizer™ Kit

The Ion Library Equalizer™ Kit is a simple and seamless bead-based solution replacing the need for library quantification and library자세히 알아보기

The Ion Library Equalizer™ Kit is a simple and seamless bead-based solution replacing the need for library quantification and library dilutions for library normalization as required for any next generation sequencing workflow. The Ion Library Equalizer™ Kit helps reduce labor, reagent costs, and further simplifies the Ion semiconductor sequencing workflow for high sample-throughput or barcoded projects, as well as single or low sample number projects.

The kit workflow consists of three simple steps:

1) Library amplification with Equalizer™ Primers
2) Library capture onto Equalizer™ Beads
3) Heat elution of the normalized library using a specially formulated Equalizer™ Elution Buffer

Simple Workflow
• Seamlessly integrates into your existing library preparation workflow
• Reduced pipetting compared to the Agilent 2100 Bioanalyzer™-based workflow
• Faster workflow in high-throughput applications-no need for DNA quantification, calculations, and dilution of DNA libraries
• Minimizes library-to-library variability

Cost Effective
• No additional capital equipment required
• Less expensive than Agilent 2100 Bioanalyzer™-based workflow (up to 10 samples)
• Reduced AMPure™ XP reagent usage compared to Agilent 2100 Bioanalyzer™-based workflow
• Long shelf life: up to 18 months
• Convenient 96-reaction kit helps reduce ordering frequency

Flexible
• Compatible with all Ion DNA library kits including Ion AmpliSeq™ DNA panels, Ion fragment library kits, and Ion Xpress™ Barcode adaptors
• Use with a single sample or in a 96-well plate format
• Compatible with 3rd party automated liquid handling systems for integrated library preparation and normalization

Includes:
The Ion Library Equalizer Kit includes the necessary reagents to complete 96 normalization reactions.

Required for Workflow:
Ion AmpliSeq™ Library Kit 2.0
Ion AmpliSeq™ Ready-to-Use Panel or Custom Panel designed via the Ion AmpliSeq™ Designer (www.ampliseq.com)
--OR--
Ion Plus Fragment Library Kit
--OR--
Ion Xpress™ Plus Fragment Library Kit

Optional:
Ion Xpress™ Barcode Adapters Kits

사양

제품라인Equalizer™

시작 물질DNA

형식96-well Plate

용도(애플리케이션)Amplicon Sequencing

용도(장비)Ion PGM™ System, Ion Proton™ System, Ion Personal Genome Machine (PGM™) System

라이브러리Fragment Library, Targeted Sequencing Library, Normalized Library

멀티플렉스 기능No Limit on Multiplexing

제품 유형Library Equalizer Kit

수량96 reactions

샘플 종류Any Sample Type

샘플 부피Fragment or Targeted AmpliSeq Libraries

시퀀싱 유형Epigenetic Sequencing, Genome & DNA Sequencing

종All Species

처리량Up to 10 Samples

워크플로우 단계Library Barcoding, Library Generation, Template Preparation

Unit SizeEach