Thermo Fisher Scientific Peroxidase (Horseradish) Polyclonal Antibody, Biotin
Horseradish peroxidase에 특이적인 Biotin 결합 폴리클로날 항체로 Western blot, IHC, IP 등 다양한 응용에 사용 가능. 높은 감도와 안정성을 제공하며 연구용으로 적합. 장기 보관 시 -20°C에서 보관 권장.
- 카탈로그번호
- 200-4638x (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Peroxidase (Horseradish) Polyclonal Antibody, Biotin
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:250 |
| Immunoprecipitation (IP) | 1:500–1:2,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peroxidase (Horseradish) |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid repeated freeze/thaw cycles. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Anti-Peroxidase (Horseradish) Antibody Biotin Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation, and ion exchange chromatography followed by extensive dialysis against the specified buffer.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum, as well as purified and partially purified Peroxidase (Horseradish). Cross-reactivity against peroxidase from other sources is unknown.
Target Information
Horseradish peroxidase (HRP) is an enzyme derived from the root of the horseradish plant. It catalyzes the oxidation of various substrates in the presence of hydrogen peroxide.
HRP is widely used as a detection enzyme in immunoassays such as ELISA and Western blot due to its high sensitivity and stability. It also serves as a tracer or labeling agent in enzyme-linked and fluorescent assays.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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