Thermo Fisher Scientific Galectin 3 Polyclonal Antibody
Galectin 3 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC/IF, ELISA에 사용 가능. Human, Dog, Rat 반응성. 항원 친화 크로마토그래피로 정제된 액상형 항체로, 연구용으로 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | 1 publication |
| ELISA | Assay-dependent | - |
Product Specifications
| Category | Specification |
|---|---|
| Species Reactivity | Dog, Human, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to amino acids 1–184 of Galectin 3 (Uniprot ID: P17931) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.85 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2552171 |
Product Specific Information
- Recommended positive controls: HeLa, A549, H1299, HCT116, MCF-7, Recombinant LGALS3 protein, rat colon
- Predicted reactivity: Rhesus Monkey (91%)
- Store product as a concentrated solution. Centrifuge briefly before opening the vial.
Target Information
Galectin 3 is a galactose-specific lectin that binds IgE and may mediate endothelial cell migration via α3β1 integrin and CSPG4 interaction. Together with DMBT1, it is required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus, it functions as a pre-mRNA splicing factor. It is involved in acute inflammatory responses including neutrophil activation and adhesion, monocyte/macrophage chemoattraction, opsonization of apoptotic neutrophils, and mast cell activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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