Thermo Fisher Scientific TCR DO11.10 Monoclonal Antibody (KJ1-26 (KJ126, KJI-26, KJI26)), APC, eBioscience

Applications

Flow Cytometry (Flow)

• Tested Dilution: 0.25 µg/test
• 13 Publications

Functional Assay (Functional)

• Tested Dilution: -
• 1 Publication

Product Specifications

Additional Formats

• Biotin
• FITC
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The KJ1-26 anti-idiotypic monoclonal antibody binds the T-cell receptor (TCR) expressed on DO11.10 and DO11.10.24 murine T cell hybridomas, but not on other T cell hybridomas tested.
The TCR on DO11.10 recognizes an ovalbumin peptide in the context of I-A^d major histocompatibility molecules.
This antibody has been instrumental in studying T cell immunity.

Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of DO11.10 T cell hybridoma (≤0.25 µg/test).
A test is defined as the amount of antibody used to stain cells in 100 µL volume (10⁵–10⁸ cells/test).
Titrate antibody for optimal performance.

Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

TCR DO11 (DO11.10) is a receptor found on lymphocytes of DO11.10 transgenic mice and T-cell receptors of BALB/c-derived DO11.10 and DO11.10.24 T cell hybridomas.
The DO11.10 TCR recognizes a C-terminal ovalbumin peptide (OVA 323–339) in the context of I-Ad MHC molecules.
Antibodies against DO11.10 have been key in understanding T cell activation, development, and function.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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