Thermo Fisher Scientific G-CSF Polyclonal Antibody, Biotin, PeproTech
Biotin으로 표지된 토끼 폴리클로날 항체로, 마우스 G-CSF 단백질 검출에 사용됩니다. Western blot과 ELISA에 최적화되어 있으며, 항원 친화 크로마토그래피로 정제되었습니다. 동결건조 형태로 제공되며, -20°C에서 보관 가능합니다.
- 카탈로그번호
- 500-P69BT-500UG
- 판매단위
- pk
카탈로그
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | – |
| ELISA | 0.25–1.0 µg/mL | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Murine G-CSF |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
Product Specific Information
Amino Acid Sequence of Recombinant Protein:
MVPLVTVSAL PPSLPLPRSF LLKSLEQVRK IQASGSVLLE QLCATYKLCH PEELVLLGHS LGIPKASLSG CSSQALQQTQ CLSQLHSGLC LYQGLLQALS GISPALAPTL DLLQLDVANF ATTIWQQMEN LGVAPTVQPT QSAMPAFTSA FQRRAGGVLA ISYLQGFLET ARLALHHLA.
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Murine G-CSF. Anti-Murine G-CSF-specific antibody was purified by affinity chromatography and subsequently biotinylated.
Sandwich ELISA:
To detect mG-CSF by sandwich ELISA (100 µL/well antibody solution), use 0.25–1.0 µg/mL of this antibody. When combined with PeproTech Polyclonal Anti-Murine G-CSF (500-P69) as the capture antibody, at least 0.2–0.4 ng/well of recombinant mG-CSF can be detected.
Western Blot:
For detection of mG-CSF by Western blot, use 0.1–0.2 µg/mL of this antibody. With compatible secondary reagents, the detection limit for recombinant mG-CSF is 1.5–3.0 ng/lane under both reducing and non-reducing conditions.
Packaging:
500-P69BT-1MG is supplied as 2 × 500 µg.
Target Information
G-CSF (Granulocyte Colony Stimulating Factor)
G-CSF is a naturally occurring cytokine that promotes production and antibacterial activity of neutrophils and monocytes. The human form is an 18.8 kDa protein with 175 amino acids. It is produced by monocytes, macrophages, endothelial cells, fibroblasts, astrocytes, osteoblasts, and bone marrow cells.
G-CSF influences proliferation, differentiation, and activation of hematopoietic cells and is expressed in various carcinoma and leukemia cell lines. It is encoded by two distinct DNA sequences, producing high- and low-activity isoforms.
Clinically, G-CSF is used in treatments for infections, myocardial infarction, neutropenia, sepsis, autoimmune disorders, and certain leukemias. It is also a biomarker for carcinomas and associated with Kostmann Syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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