Thermo Fisher Scientific RIP1 Polyclonal Antibody
RIP1 단백질을 인식하는 Rabbit Polyclonal 항체로, Human 시료에 반응합니다. Western blot에 적합하며, 고순도 항원 친화 크로마토그래피로 정제되었습니다. 액상 형태로 1 mg/mL 농도이며, 단기 4°C, 장기 -20°C 보관이 권장됩니다.
- 카탈로그번호
- PA534778
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific RIP1 Polyclonal Antibody
Applications
- Western Blot (WB): 1:500–1:3,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to amino acids 371–635 of RIP (Uniprot ID: Q13546) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage conditions | Store at 4°C short term; for long term, store at -20°C (avoid freeze/thaw cycles) |
| Shipping conditions | Wet ice |
| RRID | AB_2552130 |
Product Specific Information
- Recommended positive control: HUVEC
- IHC notes: Requires antigen retrieval using heat-mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Serine-threonine kinase that transduces inflammatory and cell-death signals (programmed necrosis) following death receptor ligation, activation of pathogen recognition receptors (PRRs), and DNA damage.
Upon TNFR1 activation by TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor.
Phosphorylates DAB2IP at Ser-728 in a TNF-alpha-dependent manner, activating the MAP3K5-JNK apoptotic cascade.
Ubiquitination by TRAF2 via Lys-63-linked chains enhances downstream signaling to MAPK and NF-kappa-B pathways, mediating inflammatory gene activation.
Polyubiquitinated RIP1 binds IKBKG/NEMO to activate NF-kappa-B.
Interaction with RHIM-containing adapters triggers gene activation and necrosis. RIPK1–RIPK3 association forms a necrosis-inducing complex.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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