Thermo Fisher Scientific Calsequestrin-1 Polyclonal Antibody
Calsequestrin-1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal 항체. Western blot, IHC, ICC, ELISA 등 다양한 응용에 적합. Human, Mouse, Rat 반응성. Protein A 정제, 1 mg/mL 농도, -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:300 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200–1:400 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide derived from human Calsequestrin (amino acids 1–100) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The sarcoplasmic reticulum (SR) is responsible for maintaining intracellular calcium levels in cardiac and skeletal muscle by storing and releasing calcium. Among several intralumenal SR calcium-binding proteins, calsequestrin is the most prominent. It sequesters calcium in the sarcoplasmic reticulum of muscle cells during relaxation and is localized to the junctional and corbular (terminal cisternae) SR. Calsequestrin functions to localize calcium near the junctional face of the terminal cisternae, from which calcium can be released into the cytosol via the ryanodine receptor. This protein is highly acidic and has a large capacity and moderate to low affinity for calcium.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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