Thermo Fisher Scientific CDK9 Monoclonal Antibody (K.513.1)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

-

Immunocytochemistry (ICC/IF)

1:50-1:100

-

Flow Cytometry (Flow)

1:100-1:400

-

Immunoprecipitation (IP)

1:100

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Dog, Hamster, Human, Mouse, Non-human primate, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

K.513.1

Immunogen

Synthetic peptide corresponding to residues near the carboxy terminus of human CDK9 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CDK9,uniProtId:P50750-1,ncbiNodeId:9606,antigenRange:372,antigenLength:372,antigenImageFileName:MA5-14912_CDK9_P50750-1_Rabbit.svg,antigenImageFileNamePDP:MA5-14912_CDK9_P50750-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

163 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_10986858

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

CDK9 is a serine/threonine kinase which when complexed with CyclinT1 forms an active holoenzyme complex. This complex plays a key role in regulating the eukaryotic cell cycle. CDK9 is coexpressed and copurified with CyclinT1. CDK9 regulates cytokine inducible transcription networks by facilitating promoter recognition of target transcription factors. CDK9 promotes RNA synthesis in genetic programs for cell growth, differentiation, and viral pathogenesis. The CDK9/cyclin-K complex also has kinase activity towards CTD of RNAPII and can substitute for CDK9/cyclin-T P-TEFb in vitro. Chronic activation of CDK9 causes cardiac myocyte enlargement leading to cardiac hypertrophy and confers a predisposition to heart failure.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.