Thermo Fisher Scientific Calcineurin B/PPP3R1 Monoclonal Antibody (BE2.1)
Calcineurin B/PPP3R1 단백질을 인식하는 BE2.1 클론의 마우스 단일클론 항체로, 인간 및 Xenopus 시료에 반응합니다. Western blot, IHC, ELISA에 사용 가능하며, 단백질 A/G 정제 및 PBS(pH 7.4) 용액 형태로 제공됩니다. 연구용으로만 사용됩니다.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | - |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL | - |
| ELISA | Assay-dependent | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Xenopus |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | BE2.1 |
| Immunogen | Recombinant full-length human Calcineurin B protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Calcineurin is a Ser/Thr, calcium and calmodulin-dependent protein phosphatase that plays an essential role in the T cell activation pathway. It consists of two subunits: calcineurin A (CnA), a 60 kDa catalytic subunit, and calcineurin B (CnB), a 19 kDa regulatory subunit containing four EF-hand calcium binding sites. Calcineurin-mediated dephosphorylation of the nuclear factor of activated T-cells (NF-AT) is crucial for NF-AT activation, nuclear translocation, and early gene expression in T-cells. Immunosuppressive drugs such as cyclosporin A and FK506 inhibit NF-AT activation by forming complexes that block substrate access to the catalytic domain of CnA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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