Thermo Fisher Scientific Calcineurin B/PPP3R1 Monoclonal Antibody (CALNB, 2342)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1–2 µg/mL

Product Specifications

Product Specific Information

• Immunohistochemistry (PFA fixed): Incubate antibody for 30 min at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 min.

Target Information

Calcineurin is a Ser/Thr, calcium and calmodulin-dependent protein phosphatase that plays an essential role in the T-cell activation pathway.
It consists of two subunits:

• Calcineurin A (CnA): 60 kDa catalytic subunit, homologous to protein phosphatases 1 and 2A
• Calcineurin B (CnB): 19 kDa regulatory subunit with four EF-hand calcium-binding sites

Calcineurin-mediated dephosphorylation of NF-AT is essential for its activation, nuclear translocation, and early gene expression in T-cells.
Immunosuppressive drugs such as cyclosporin A and FK506 inhibit NF-AT activation by forming complexes that bind to both CnA and CnB, blocking substrate access to CnA’s catalytic domain.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.