Thermo Fisher Scientific Phospho-ATF2 (Ser62, Ser44) Polyclonal Antibody
ATF2 단백질의 Ser62 및 Ser44 인산화 형태를 특이적으로 인식하는 다클론 항체. Western blot, IHC, ICC, IP 등 다양한 응용에 적합. 인간, 마우스, 랫트 시료에 반응. PBS/glycerol buffer에 보관되며 -20°C에서 안정적.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human ATF2 (Accession P15336), corresponding to amino acid residues around phosphorylated Ser62. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816275 |
Product Specific Information
This antibody detects endogenous levels of ATF2 only when phosphorylated at Serine 62 or 44.
Target Information
The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and belongs to the ATF/CREB family of leucine zipper proteins.
ATF-2 interacts with various viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways.
Cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulates ATF-2 transcriptional activity.
Activation occurs via phosphorylation at Thr69 and Thr71, mediated by SAPK and p38 MAPK both in vitro and in transfected cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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