Thermo Fisher Scientific Desmoglein 3 Recombinant Mouse Monoclonal Antibody (AK23)

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

1:100

-

Flow Cytometry (Flow)

1:100

-

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

AK23

Immunogen

The original antibody was generated by immunizing Dsg3 knockout mice with recombinant mouse Dsg3-His in complete Freunds adjuvant subcutaneously. if (typeof window.$mangular ===undefined|| !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson =[{targetFamily:Desmoglein 3,uniProtId:O35902-1,ncbiNodeId:10090,antigenRange:1-993,antigenLength:993,antigenImageFileName:MA5-51877\_Desmoglein\_3\_O35902-1\_House\_mouse.svg,antigenImageFileNamePDP:MA5-51877\_Desmoglein\_3\_O35902-1\_House\_mouse\_PDP.jpeg,sortOrder:2}]; $mangular.isB2BCMGT = ``false``; $mangular.isEpitopesModalImageMultiSizeEnabled =true`;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.02% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

Product Specific Information

This antibody binds mouse Dsg3 and also shows reactivity for human Dsg3. It does not show any affinity towards Dsg1. AK23 recognizes a calcium-dependent conformational epitope on Dsg3, which consisted of the V3, K7, P8, and D59 Dsg3-specific residues that formed the adhesive interface between juxtaposed Dsg.

Target Information

Desmoglein 3 (Dsg3), also known as Cadherin family member 6 (CDHF6), is a member of the desmosomal cadherin family and plays a critical role in cell-cell adhesion. It is predominately expressed in stratified squamous epithelium, including the skin, oral mucosa, and esophagus. Desmoglein 3 has been characterized as the auto-antigen of pemphigus vulgaris (PV), a life-threatening autoimmune disease. Binding of anti-Desmoglein 3 autoantibodies causes the loss of cell-cell adhesion, which clinically manifests as blisters on the skin and oral mucosa, and histologically as suprabasilar acantholysis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.