Thermo Fisher Scientific SMURF2 Polyclonal Antibody
SMURF2 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF 등 다양한 응용에 적합. 항원 친화 크로마토그래피로 정제된 고품질 제품으로 인간 SMURF2 단백질에 반응. 연구용으로만 사용 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human SMURF2. Recombinant protein control fragment (Product #RP-105534) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792015 |
Product Specific Information
Immunogen sequence:
LSCFVDENTP ISGTNGATCG QSSDPRLAER RVRSQRHRNY MSRTHLHTPP D
Target Information
SMURF2 is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.
This protein interacts with SMAD1, SMAD2 and SMAD7 to trigger their ubiquitination and proteasome-dependent degradation. It enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2.
Coexpression of SMURF2 with SMAD1 results in a considerable decrease in the steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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