Thermo Fisher Scientific SKA3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

-

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000-1:2,500

-

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human SKA3. Recombinant protein control fragment (Product #RP-97983). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SKA3,uniProtId:Q8IX90-1,ncbiNodeId:9606,antigenRange:109-195,antigenLength:412,antigenImageFileName:PA5-58722_SKA3_Q8IX90-1_Rabbit.svg,antigenImageFileNamePDP:PA5-58722_SKA3_Q8IX90-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.55 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2647326

Product Specific Information

Immunogen sequence: NSVHEQEAIN SDPELSNCEN FQKTDVKDDL SDPPVASSCI SEKSPRSPQL SDFGLERYIV SQVLPNPPQA VNNYKEEPVI VTPPTKQ

Highest antigen sequence identity to the following orthologs: Mouse - 54%, Rat - 44%.

Target Information

Upon entry into mitosis, the cell`s microtubule (MT) network forms the mitotic spindle, allowing the segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin termed kinetochores (KT) are essential for the proper attachment of the chromosomes to the spindle MTs. A recently discovered spindle and kinetochore complex, comprised of proteins SKA1, SKA2, and SKA3, has been found to be required for stable KT-MT interactions and timely anaphase onset. Like with SKA1 or SKA2, depletion of SKA3 by siRNA delays anaphase transition, resulting in a prolonged a metaphase-like state. These SKA3-depleted cells accumulate high levels of the checkpoint protein Bub1 at kinetochores, suggesting the SKA complex plays a key role in spindle checkpoint silencing and the maintenance of chromosome cohesion in mitosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.