Thermo Fisher Scientific CCR2 (extracellular) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:150

-

Flow Cytometry (Flow)

2.5 µg/5x10^5 cells

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

RNTNESGEEVTTFFD(C), corresponding to amino acid residues 11-25 of human CCR2, Extracellular, N-terminus if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CCR2 (CD192),uniProtId:P41597-1,ncbiNodeId:9606,antigenRange:11-25,antigenLength:374,antigenImageFileName:ACR-022-200UL_CCR2_CD192_P41597-1_Rabbit.svg,antigenImageFileNamePDP:ACR-022-200UL_CCR2_CD192_P41597-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.8 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Information

CCR2, a chemokine receptor, is a regulator of monocyte/macrophage traffickings and is upregulated in response to inflammation. Resistance to HIV-1 infection or delayed progression to AIDS may be linked to CCR2 polymorphisms. The ligand for CCR2 is monocyte chemoattractant protein (MCP-1), a chemokine produced by endothelial cells, smooth muscle cells, and macrophages. Activation of monocytes by MCP-1 may mediate the monocytic infiltration of tissues in atherosclerosis and other inflammatory disorders including rheumatoid arthritis, alveolitis, and tumor infiltration. Two isoforms are produced by alternative splicing. CCR2 has been reported to be expressed in a wide variety of tissues, including blood, brain, heart, kidney, liver, lung, ovary, pancreas, spinal cord, spleen, thymus, and vessel. Increased expression of CCR2 has been documented in CD3+ lymphocytes in rheumatoid arthritis. ESTs have been isolated from liver/spleen and prostate libraries.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.