Thermo Fisher Scientific V5 Tag Monoclonal Antibody (SV5-Pk1)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

View 697 publications 697 publications

Immunohistochemistry (IHC)

-

View 78 publications 78 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 1 publication 1 publication

Immunohistochemistry (PFA fixed) (IHC (PFA))

-

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100-1:500

View 182 publications 182 publications

Flow Cytometry (Flow)

-

View 20 publications 20 publications

ELISA (ELISA)

Assay-dependent

View 5 publications 5 publications

Immunoprecipitation (IP)

Assay-dependent

View 157 publications 157 publications

ChIP assay (ChIP)

-

View 38 publications 38 publications

Functional Assay (Functional)

-

View 1 publication 1 publication

in situ PLA (PLA)

-

View 6 publications 6 publications

Dot blot (DB)

-

View 3 publications 3 publications

Inhibition Assays (IA)

-

View 1 publication 1 publication

RNA Immunoprecipitation (RIP)

-

View 3 publications 3 publications

In vitro Assay (IV)

-

View 2 publications 2 publications

GST Pull Down (GPD)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 19 publications 19 publications

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

SV5-Pk1

Immunogen

V5 synthetic peptide: Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr-.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

purified

Storage buffer

PBS

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2556564

Product Specific Information

NOTE: R960-25 was previously sold under product #46-0705. When re-ordering, please use #R960-25.
R960CUS was previously sold under product #46-1157. When re-ordering, please use #R960CUS.

This antibody recognizes amino acid sequence: -Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr-.

This antibody is functionally tested against 20 ng of an E. coli expressed fusion protein containing a V5 epitope using a chemiluminescent substrate at a 1 minute exposure. This antibody has also been tested in Western blot against 25 ng of recombinant Positope™ protein. The Positope™ control protein is a 53 kDa recombinant protein that contains seven epitope tags, including His (C-term), HisG, c-myc, and V5. Low background was observed using chemiluminescent or alkaline phosphatase reagents for detection. For Western blot, dilute in PBS or Tris-Buffered Saline (TBS) containing 0.05% Tween-20 and 5% nonfat, dry milk (PBSTM or TBSTM).

Using chemiluminescence as the detection method, no cross-reactivity has been observed in bacterial lysates. In mammalian lysates, a few cross-reactive proteins have been observed upon overexposure of blots.

This antibody has also been used successfully to immunoprecipitate fusion proteins that contain theV5 epitope.

Target Information

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.