Thermo Fisher Scientific V5 Tag Monoclonal Antibody (SV5-Pk1)
V5 태그 인식용 단클론 항체로, 다양한 단백질 발현 분석에 사용됩니다. Western blot, ICC/IF, IP 등 다중 응용에 적합하며, 낮은 백그라운드와 높은 특이성을 제공합니다. 마우스 IgG2a 형식의 정제 항체로 연구용에 적합합니다.
- 카탈로그번호
- R960xxx (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific V5 Tag Monoclonal Antibody (SV5-Pk1)
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 | 697 |
| Immunohistochemistry (IHC) | – | 78 |
| Immunohistochemistry (Paraffin) (IHC (P)) | – | 1 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | – | 2 |
| Immunohistochemistry (Frozen) (IHC (F)) | – | 1 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 | 182 |
| Flow Cytometry (Flow) | – | 20 |
| ELISA | Assay-dependent | 5 |
| Immunoprecipitation (IP) | Assay-dependent | 157 |
| ChIP assay (ChIP) | – | 38 |
| Functional Assay | – | 1 |
| in situ PLA (PLA) | – | 6 |
| Dot blot (DB) | – | 3 |
| Inhibition Assays (IA) | – | 1 |
| RNA Immunoprecipitation (RIP) | – | 3 |
| In vitro Assay (IV) | – | 2 |
| GST Pull Down (GPD) | – | 1 |
| Miscellaneous PubMed (Misc) | – | 19 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Published Species | Tag |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SV5-Pk1 |
| Immunogen | V5 synthetic peptide: Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr- |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Purified |
| Storage Buffer | PBS |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| RRID | AB_2556564 |
Product Specific Information
- R960-25 was previously sold under product #46-0705. When re-ordering, please use #R960-25.
- R960CUS was previously sold under product #46-1157. When re-ordering, please use #R960CUS.
This antibody recognizes the amino acid sequence: Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr-.
It is functionally tested against 20 ng of an E. coli-expressed fusion protein containing a V5 epitope using a chemiluminescent substrate at a 1-minute exposure. It has also been tested in Western blot against 25 ng of recombinant Positope™ protein (53 kDa recombinant protein with seven epitope tags including His, HisG, c-myc, and V5).
Low background was observed using chemiluminescent or alkaline phosphatase reagents. For Western blot, dilute in PBS or Tris-Buffered Saline (TBS) containing 0.05% Tween-20 and 5% nonfat dry milk (PBSTM or TBSTM).
No cross-reactivity has been observed in bacterial lysates when using chemiluminescence detection. In mammalian lysates, a few cross-reactive proteins may appear upon overexposure of blots.
This antibody has also been successfully used to immunoprecipitate fusion proteins containing the V5 epitope.
Target Information
Epitope tags provide a method to localize gene products in various cell types, study protein topology and complexes, identify associated proteins, and characterize newly identified or low-abundance proteins when specific antibodies are unavailable.
The V5 epitope tag is derived from the P and V proteins of the paramyxovirus SV5. It is typically used with the full 14 amino acid sequence (GKPIPNPLLGLDST) or a shorter 9 amino acid version (IPNPLLGLD).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.