Thermo Fisher Scientific FALZ/BPTF Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000–1:10,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:200–1:1,000

Immunoprecipitation (IP)

• Tested Dilution: 2–10 µg/mg lysate

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• Application Note: For IHC, epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.

Target Information

BPTF (Bromodomain and PHD Domain Transcription Factor) is the largest subunit of the ATP-dependent chromatin-remodelling complex NURF (Nucleosome Remodelling Factor).
NURF catalyzes ATP-dependent nucleosome sliding and facilitates transcription. BPTF recognizes histone H3 tails tri-methylated at K4, marking transcriptional start sites of active genes. It also binds H3 di-methylated at K4.

BPTF is crucial in early embryonic development, possibly through Smad pathway regulation. It is expressed at low levels in adult brain and spinal cord but increases in neurodegenerative diseases, including Alzheimer’s disease.
Abundantly expressed in fetal brain and throughout gray and white matter of the developing spinal cord (18–22 gestational weeks).
Tissue specificity: Ubiquitously expressed, highest in testis; also present in kidney, liver, and brain, with highest levels in motor cortex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.