Thermo Fisher Scientific CD177 Monoclonal Antibody (MEM-166 (MEM166)), eFluor 660, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.015 µg)/test

-

Product Specifications

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 660, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MEM-166 (MEM166)

Conjugate

eFluor™ 660 eFluor™ 660 eFluor™ 660

View additional formats

• Unconjugated
• Request custom conjugation

Excitation/Emission Max

651/668 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Wet ice

RRID

AB_2574188

Product Specific Information

Description: The MEM-166 monoclonal antibody reacts with human CD177, a 58-64 kDa GPI-anchored glycoprotein. The human CD177 locus codes for at least two alleles, NB1 and PRV-1, which differ by only 4 amino acids. CD177 is expressed on granulocytes, early erythroblasts, megakaryocytes, promyelocytes and myelocytes. CD177 mRNA levels are elevated in several different myeloproliferative disorders including polycythemia vera, and antibodies to CD177 are involved in neonatal alloimmune neutropenia, autoimmune drug induced neutropenia, and graft failure following marrow transplantation.

Applications Reported: This MEM-166 (MEM166) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This MEM-166 (MEM166) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.015 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD177 (NB1/HNA-2a and PRV-1 form) is a GPI-anchored glycoprotein present mainly on neutrophils. Its plasma membrane expression is increased during pregnancy and and inflammation or after G-CSF application. Ligand of CD177 has been identified as CD31 (PECAM-1). CD177 participates in neutrophil transmigration and seems to be also a pro-proliferative molecule. The antibodies against CD177 can be involved in neonatal alloimmune neutropenia (NAN).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.