Thermo Fisher Scientific Cit-Histone H3 (Arg2, Arg8, Arg17) Polyclonal Antibody
Western blot, IHC, ICC, IP 등에 사용 가능한 다중 시트룰린화 히스톤 H3(Arg2, Arg8, Arg17) 특이 폴리클로날 항체. 토끼 유래 IgG로 항원 친화 크로마토그래피 정제. 핵 단백질 검출용, 4°C 단기 보관 및 -20°C 장기 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
| Immunoprecipitation (IP) | 2–5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Chicken, Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide containing the N-terminus of human histone H3 with citrulline modification at Arg2, Arg8, and Arg17 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: 3T3 Nuclear Extract
- Cellular location: Nuclear
Target Information
Histones are basic nuclear proteins that, together with DNA, form the nucleosome structure in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of the four core histones (H2A, H2B, H3, and H4).
Methylation of specific residues in histone tails is a key modification regulating epigenetic transitions in chromatin.
- Methylation of H3 on lysine 4, 36, and 79 is linked with gene activation.
- Methylation of H3 on lysines 9, 27 and H4 on lysine 20 is associated with heterochromatin and gene repression.
Modified lysine residues can exist in mono-, di-, or tri-methylated states, while arginine residues can be mono- or di-methylated.
Histone H3 Lys4 trimethylation (H3-K4me3) is a conserved mark of actively transcribed chromatin.
This antibody specifically recognizes histone H3 citrullinated at Arg2, Arg8, and Arg17. The sequence is conserved among mammals and various other species including Drosophila, Arabidopsis, Chicken, and Xenopus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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