Thermo Fisher Scientific Cit-Histone H3 (Arg2, Arg8, Arg17) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-1 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

2-10 µg/mL

-

Immunocytochemistry (ICC/IF)

5 µg/mL

-

Immunoprecipitation (IP)

2-5 µg/mL

-

Product Specifications

Species Reactivity

Bovine, Chicken, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide containing N-terminus of human histone H3 with citrulline modification at 2/8/17 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:H3R2R8R17cit,uniProtId:P68431-1,ncbiNodeId:9606,antigenRange:1,antigenLength:136,antigenImageFileName:630-180ABBOMAX_H3R2R8R17cit_P68431-1_Rabbit.svg,antigenImageFileNamePDP:630-180ABBOMAX_H3R2R8R17cit_P68431-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with proprietary stabilizer

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Positive control: 3T3 Nuclear Extract

Cellular location: Nuclear.

Target Information

Histones are basic nuclear proteins that together with DNA make up the nucleosome structure in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The methylation of specific residues in the histone tails is a central modification for regulatingepigenetic transitions in chromatin. Whereas methylation of histone H3 on lysine 4, 36, and 79 has been linked with gene activation, methylation of H3 on lysines 9, 27 and histone H4 on lysine 20 is associated with heterochromatin and some repressed genes with euchromatin. Modified lysine residues can exist in a mono-, di-, or tri-methylated state, while the arginine residues can be mono- or di-methylated. Histone H3 Lys4 trimethylation (H3-K4me3) is a conserved mark of actively transcribed chromatin. This antibody is specific for histone H3 tri-methylated at K27. The sequence is found in all mammals and a wide range of species, including D. melanogaster, Arabidopsis, Chicken and Xenopus. The antibody will react with any of the above species where the trimethylation modification is present.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.