Thermo Fisher Scientific KEAP1 Recombinant Rabbit Monoclonal Antibody (PSH0-90)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000

-

Flow Cytometry (Flow)

1:500-1:1,000

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

PSH0-90

Immunogen

Recombinant protein within human keap1 aa 275-624/624 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:KEAP1,uniProtId:Q14145-1,ncbiNodeId:9606,antigenRange:275-624,antigenLength:624,antigenImageFileName:MA5-49609_KEAP1_Q14145-1_Rabbit.svg,antigenImageFileNamePDP:MA5-49609_KEAP1_Q14145-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.1% BSA, 40% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3094136

Product Specific Information

Sequence Similarities: 98% Mouse/Rat.

Tissue Specificity: Broadly expressed, with highest levels in skeletal muscle.

Positive Control: HepG2 cell lysate, HeLa cell lysate, MCF7 cell lysate, A431 cell lysate, Jurkat cell lysate, HEK-293 cell lysate, Daudi cell lysate, A549 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse kidney tissue, rat kidney tissue, PC-12.

Subcellular Location: Cytoplasm, Nucleus.

Predicted band size: 70 kDa.

Target Information

This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.