Thermo Fisher Scientific AHNAK Recombinant Rabbit Monoclonal Antibody (PSH01-10)
AHNAK 단백질을 표적으로 하는 Thermo Fisher의 재조합 토끼 단클론 항체. WB, IHC, ICC/IF에 적합하며 인간, 마우스, 랫트 반응성. 고순도 Protein A 정제, 안정적 액상 형태로 장기 보관 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:5,000–1:20,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:250 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | PSH01-10 |
| Immunogen | Recombinant protein within human AHNAK aa 451–900/5,890 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 0.1% BSA, 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3092422 |
Product Specific Information
- Sequence Similarities: 100% Mouse/Rat
- Tissue Specificity: Low tissue specificity
- Positive Control: Human lung carcinoma tissue, human brain tissue, mouse brain tissue, rat brain tissue, A431, HeLa, NIH/3T3
- Subcellular Location: Nucleus
- Predicted band size: 629 kDa
Target Information
AHNAK1 (Desmoyokin) is a large (~700 kDa) scaffold protein that translocates to the plasma membrane after an increase in extracellular calcium or upon protein kinase C activation, regulating calcium influx via L-type Ca²⁺ channels. It is involved in signal transduction processes influencing cell differentiation and proliferation.
In calcium-dependent intercellular contacts, AHNAK1 forms multimeric complexes at the plasma membrane with actin and annexin 2/S100A10 assemblies, contributing to membrane architecture organization.
In epithelial cells, AHNAK1 is cytoplasmic or membrane-associated, while in non-epithelial cells it is predominantly nuclear, exhibiting weak DNA-binding activity and association with the DNA-ligase IV–XRCC4 complex.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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