Thermo Fisher Scientific AHNAK Recombinant Rabbit Monoclonal Antibody (PSH01-10)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:5,000-1:20,000

-

Immunocytochemistry (ICC/IF)

1:100-1:250

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

PSH01-10

Immunogen

Recombinant protein within human AHNAK aa 451-900/5,890. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:AHNAK,uniProtId:Q09666-1,ncbiNodeId:9606,antigenRange:451-900,antigenLength:5890,antigenImageFileName:MA5-49610_AHNAK_Q09666-1_Rabbit.svg,antigenImageFileNamePDP:MA5-49610_AHNAK_Q09666-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.1% BSA, 40% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3092422

Product Specific Information

Sequence Similarities: 100% Mouse/Rat.

Tissue Specificity: Low tissue specificity.

Positive Control: Human lung carcinoma tissue, human brain tissue, mouse brain tissue, rat brain tissue, A431, HeLa, NIH/3T3.

Subcellular Location: Nucleus.

Predicted band size: 629 kDa.

Target Information

AHNAK1 (Desmoyokin) is a large (700 kDa) scaffold protein that translocates to the plasma membrane after an increas of extracellular calcium level or upon proteinkinase C activation and regulates extracellular calcium influx mediated by L-type Ca2+ channels. AHNAK1 has been implicated in diverse signal transduction proceses affecting cell differentiation and proliferation. In response to calcium-dependent intercellular contacts AHNAK1 forms multimeric complexes in the plasma membrane, connected with actin and annexin 2/S100A10 assemblies and is thus involved in organization of the plasma membrane architecture. In epithelial cells, AHNAK1 is localized in cytoplasm or is membrane-associated, but in cells of nonepithelial origin AHNAK1 is predominantly nuclear; it has a weak DNA-binding activity and associates with the DNA-ligase IV-XRCC4 complex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.