Thermo Fisher Scientific SATB1 Monoclonal Antibody (OTI11E8), TrueMAB
SATB1 단백질을 인식하는 Mouse Monoclonal Antibody로 Western blot, IHC, ICC, Flow Cytometry에 사용 가능. Human 시료에 반응하며, Lyophilized 형태로 제공. 고순도 Affinity chromatography 정제, PBS buffer(8% trehalose) 보존.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI11E8 |
| Immunogen | Full length human recombinant protein of human SATB1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
SATB1 (DNA-binding protein SATB1) is a crucial silencing factor involved in X inactivation mediated by Xist RNA during embryogenesis and in lymphoma.
It binds to AT-rich DNA sequences and nuclear matrix-associated regions, recognizing the sugar-phosphate backbone of double-stranded DNA.
SATB1 functions as a transcriptional repressor that regulates nuclear and viral gene expression through chromatin-loop remodeling.
It modulates genes essential for T-cell maturation and may influence HIV-1 integration and transcriptional regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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