Thermo Fisher Scientific Phospho-STK29 (Thr260) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human BRSK2 (Phospho-Thr260) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:STK29,uniProtId:Q8IWQ3-1,ncbiNodeId:9606,antigenRange:243-272,antigenLength:736,antigenImageFileName:PA5-64568_STK29_Q8IWQ3-1_Rabbit.svg,antigenImageFileNamePDP:PA5-64568_STK29_Q8IWQ3-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2663647

Product Specific Information

Phospho-STK29 (Thr260) Polyclonal Antibody detects endogenous levels of STK29 only when phosphorylated at Thr260.

Target Information

BRSK2 is a brain-selective serine/threonine kinase 2 that is mainly expressed in the brain, with weaker expression in testis and pancreas. BRSK2 expressed in insect cells specifically phosphorylates WEE1A, CDC25C and CDC25B in an in vitro assay. DNA damage induced by ultraviolet (UV) irradiation or methyl methane sulfonate, but not by ionizing radiation, enhanced endogenous BRSK2 kinase activity in a caffeine-sensitive manner and caused translocation of BRSK2 from the cytoplasm to the nucleus. Overexpression of BRSK2 in HeLa cells results in increased phosphorylation of CDC25C. DNA damage induced by ultraviolet (UV) irradiation or methyl methane sulfonate, but not by ionizing radiation, enhances endogenous BRSK2 kinase activity in a caffeine-sensitive manner and causes translocation of BRSK2 from the cytoplasm to the nucleus. Overexpression of BRSK2 induces G2/M arrest in HeLa cells. Small interfering RNA against BRSK2 partly abrogates UV-induced G2/M arrest. BRSK2 may act as a checkpoint kinase upon DNA damage induced by UV irradiation or methyl methane sulfonate.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.