Thermo Fisher Scientific TRIM28 Monoclonal Antibody (20C1)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

2 µg/mL

View 3 publications 3 publications

Immunoprecipitation (IP)

Assay-dependent

-

ChIP assay (ChIP)

Assay-dependent

View 1 publication 1 publication

in situ PLA (PLA)

-

View 1 publication 1 publication

Gel Shift (GS)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

20C1

Immunogen

Recombinant protein corresponding to residues 60-383 of the human TIF1 beta protein. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:TRIM28,uniProtId:Q13263-1,ncbiNodeId:9606,antigenRange:60-383,antigenLength:835,antigenImageFileName:MA1-2023_TRIM28_Q13263-1_House_mouse.svg,antigenImageFileNamePDP:MA1-2023_TRIM28_Q13263-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2209892

Product Specific Information

MA1-2023 detects human and mouse TIF1 beta.

MA1-2023 has been used successfully in Western blot, and immunofluorescence procedures. By Western blot this antibody detects ~100 kDa protein representing TIF1 beta in gamma irradiated HeLa cell lysate. In immunofluorescence procedures, MA1-2023 recognizes TIF1 beta in gamma irradiated HeLa cells.

The MA1-2023 immunogen is recombinant protein corresponding to residues 60-383 of the human TIF1 beta protein.

Target Information

The KRAB (Kruppel-associated box) domain is about 45 amino acids in length and is a transcriptional repression domain found in numerous transcription factors. There have been identified over 220 KRAB-zinc finger protein (KRAB-ZFP) genes in the human genome. These proteins functionally repress transcription via specific interactions with KAP-1 (KRAB-associated protein 1). KAP-1 is an 835 amino acid polypeptide that contains a RING finger, B boxes, and a PHD finger. KAP-1 has been shown to form complexes with KRAB-domain transcription factors and increase the efficiency with which they mediate repression. KAP-1 has also been shown to directly interact with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel-associated box-containing zinc finger protein 1). KAP-1 directly targets KRAZ1 to the foci of centromeric heterochromatin containing HP1alpha, thus helping to regulate transcriptional repression. Studies have shown that KAP-1 mutants with the ability to bind KRAB but unable to bind HP1 leads to random distribution of KRAZ1 and strong transcriptional activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.