Thermo Fisher Scientific TRIM28 Monoclonal Antibody (20C1)
TRIM28 단백질을 인식하는 마우스 모노클로날 항체로 Western blot, IHC, ICC/IF 등 다양한 응용에 사용 가능. 인간 및 마우스 시료에 반응하며, Protein G로 정제된 액상 형태. -20°C에서 보관하며 반복 동결/해동은 피해야 함.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–3 µg/mL | View 2 publications |
| Immunohistochemistry (IHC) | – | View 2 publications |
| Immunocytochemistry (ICC/IF) | 2 µg/mL | View 3 publications |
| Immunoprecipitation (IP) | Assay-dependent | – |
| ChIP assay (ChIP) | Assay-dependent | View 1 publication |
| in situ PLA (PLA) | – | View 1 publication |
| Gel Shift (GS) | Assay-dependent | – |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 20C1 |
| Immunogen | Recombinant protein corresponding to residues 60–383 of the human TIF1 beta protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2209892 |
Product Specific Information
MA1-2023 detects human and mouse TIF1 beta.
It has been used successfully in Western blot and immunofluorescence procedures.
By Western blot, this antibody detects a ~100 kDa protein representing TIF1 beta in gamma-irradiated HeLa cell lysate.
In immunofluorescence, MA1-2023 recognizes TIF1 beta in gamma-irradiated HeLa cells.
The immunogen is a recombinant protein corresponding to residues 60–383 of the human TIF1 beta protein.
Target Information
The KRAB (Kruppel-associated box) domain (~45 amino acids) functions as a transcriptional repression domain found in numerous transcription factors.
Over 220 KRAB-zinc finger protein (KRAB-ZFP) genes exist in the human genome. These repress transcription via interaction with KAP-1 (KRAB-associated protein 1), an 835 amino acid polypeptide containing RING finger, B boxes, and a PHD finger.
KAP-1 forms complexes with KRAB-domain transcription factors, enhancing repression efficiency, and interacts directly with HP1 (heterochromatin protein 1) and KRAZ1.
KAP-1 targets KRAZ1 to centromeric heterochromatin foci containing HP1alpha, thereby regulating transcriptional repression.
Mutations that disrupt HP1 binding lead to random KRAZ1 distribution and strong transcriptional activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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