Thermo Fisher Scientific FnCpf1 Monoclonal Antibody (14H3), GenCRISPR

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

-

Immunocytochemistry (ICC/IF)

0.2-1 µg/mL

-

ELISA (ELISA)

0.005-1 µg/mL

-

Product Specifications

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

14H3

Immunogen

Recombinant Francisella tularensis subsp. novicida U112 Cpf1 (FnCpf1)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Wet ice

Product Specific Information

Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.

Cpf1-containing CRISPR-Cas loci of Francisella tularensis subsp. novicida U112 encode functional defense systems capable of mediating plasmid interference in bacterial cells guided by the CRISPR spacers. Unlike Cas9 systems, Cpf1-containing CRISPR systems have three features: First, Cpf1-associated CRISPR arrays are processed into mature crRNAs without the requirement of an additional trans-activating crRNA (tracrRNA). Second, Cpf1-crRNA complexes efficiently cleave target DNA proceeded by a short T-rich protospacer adjacent motif (PAM), in contrast to the G-rich PAM following the target DNA for Cas9 systems. Third, Cpf1 introduces a staggered DNA double stranded break with a 4 or 5-nt 5` overhang.

Target Information

Clustered Regularly Interspaced Short Palindromic Repeats from Prevotella and Francisella 1 or CRISPR/Cpf1 is a DNA-editing technology analogous to the CRISPR/Cas9 system. Cpf1 is an RNA-guided endonuclease of a class II CRISPR/Cas system. This acquired immune mechanism is found in Prevotella and Francisella bacteria.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.