Thermo Fisher Scientific HRX Monoclonal Antibody (10F8D7)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

-

ELISA (ELISA)

1:10,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

10F8D7

Immunogen

Purified recombinant fragment of MLL (aa3751-3968) expressed in E. Coli. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HRX,uniProtId:Q03164-1,ncbiNodeId:9606,antigenRange:3751-3968,antigenLength:3969,antigenImageFileName:MA5-15380_HRX_Q03164-1_House_mouse.svg,antigenImageFileNamePDP:MA5-15380_HRX_Q03164-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_10985776

Product Specific Information

MA5-15380 targets MLL in indirect ELISA, IHC and WB applications and shows reactivity with Human samples.

The MA5-15380 immunogen is purified recombinant fragment of MLL (aa3751-3968) expressed in E. Coli.

Target Information

KMT2A is a histone methyltransferase that plays an essential role in early development and hematopoiesis. It is a catalytic subunit of the MLL1/MLL complex, a multiprotein complex that mediates both methylation of Lys-4 of histone H3 (H3K4me) complex and acetylation of Lys-16 of histone H4 (H4K16ac). In the MLL1/MLL complex, KMT2A specifically mediates H3K4me, a tag for epigenetic transcriptional activation. KMT2A has weak methyltransferase activity by itself, and requires other components of the MLL1/MLL complex to obtain full methyltransferase activity. It has no activity toward histone H3 phosphorylated on Thr-3, less activity toward H3 dimethylated on Arg-8 or Lys-9, and has higher activity toward H3 acetylated on Lys-9. Required for transcriptional activation of HOXA9 and promotes PPP1R15A-induced apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.