
Thermo Fisher Scientific Rat anti-Mouse IgG1 (Heavy chain) Secondary Antibody, HRP
Rat anti-Mouse IgG1(H chain) monoclonal HRP-conjugated secondary antibody for WB and ELISA. High specificity for murine IgG1, no cross-reactivity with other subclasses. Affinity-purified, 1 mg/mL liquid form, ideal for sensitive detection and signal am...
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Applications
Tested Dilution
- Western Blot (WB): 1:1,000–1:10,000
- ELISA: 500 ng/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG1 |
| Class | Monoclonal |
| Type | Secondary Antibody |
| Clone | LO-MG1-2 |
| Immunogen | Monoclonal mouse IgG1 |
| Conjugate | HRP |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 50% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2538269 |
| Target | IgG1 (Heavy chain) |
| Antibody Form | Whole Antibody |
Additional Formats
- FITC conjugate available
- Custom conjugation requests accepted
Product Specific Information
This antibody can be used in direct ELISA or as a detection reagent in sandwich ELISA together with MA5-16782 as the capture antibody.
Rat anti-Mouse IgG1 Heavy Chain antibody, clone LO-MG1-2, specifically recognizes murine IgG1 and does not bind other mouse immunoglobulin classes or subclasses.
Target Information
Anti-Mouse secondary antibodies are affinity-purified and characterized for specificity toward mouse immunoglobulins.
They are valuable for detection, sorting, or purification of target proteins and support multiple detection systems (HRP, AP, fluorescence).
Secondary antibodies enhance sensitivity via signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically, they are generated by immunizing host animals with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, fragmentation, label conjugation) to yield highly specific reagents.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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