Thermo Fisher Scientific KCC2 Monoclonal Antibody (N1/12), FITC
KCC2 단백질을 검출하기 위한 FITC 결합 단일클론 항체로, 인간·마우스·랫트 시료에 반응합니다. WB, IHC, ICC/IF, IP 등 다양한 응용에 적합하며, 고순도 Protein G 정제 및 형광 특성(Ex/Em 498/517 nm)을 제공합니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific KCC2 Monoclonal Antibody (N1/12), FITC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:300 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N1/12 |
| Immunogen | Fusion protein amino acids 932–1043 corresponding to rat KCC2 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931950 |
Additional Formats
- Unconjugated (MA5-27610)
- APC (MA5-45495)
- PE (MA5-45498)
- PerCP (MA5-45497)
- Custom conjugation available upon request
Product Specific Information
1 µg/mL of MA5-45496 was sufficient for detection of KCC2 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Detects approximately 140 kDa.
This antibody was formerly sold as clone S1-12.
Target Information
KCC2 mediates electroneutral potassium-chloride cotransport in mature neurons. Transport occurs under isotonic conditions but is activated 20-fold by cell swelling. It plays an important role in Cl⁻ homeostasis in neurons.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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