Thermo Fisher Scientific KCC2 Monoclonal Antibody (N1/12), PerCP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (IHC)

1:300

-

Immunocytochemistry (ICC/IF)

1:100

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

N1/12

Immunogen

Fusion protein amino acids 932-1043 corresponding to rat KCC2 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:KCC2,uniProtId:Q63633-1,ncbiNodeId:10114,antigenRange:932-1043,antigenLength:1139,antigenImageFileName:MA5-45497_KCC2_Q63633-1_House_mouse.svg,antigenImageFileNamePDP:MA5-45497_KCC2_Q63633-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

PerCP PerCP PerCP

View additional formats

• Unconjugated
• APC
• FITC
• PE
• Request custom conjugation

Excitation/Emission Max

482/675 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2931951

Product Specific Information

1 µg/mL of MA5-45497 was sufficient for detection of KCC2 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 140kDa.

This antibody was formerly sold as clone S1-12.

Target Information

Mediates electroneutral potassium-chloride cotransport in mature neurons. Transport occurs under isotonic conditions, but is activated 20-fold by cell swelling. Important for Cl(-) homeostasis in neurons.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.