Thermo Fisher Scientific CD45.1 Monoclonal Antibody (A20), Brilliant Ultra Violet 661, eBioscience

Thermo Fisher Scientific CD45.1 Monoclonal Antibody (A20), Brilliant Ultra Violet 661, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 1.0 µg/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 532
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395
• Brilliant UV 563
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 605
• Brilliant Violet 650
• Brilliant Violet 711
• Brilliant Violet 786
• eFluor 450
• eFluor 506
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 610-30S
• NovaFluor Blue 660-40S
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Request custom conjugation available

Product Specific Information

The A20 monoclonal antibody reacts with the mouse CD45 molecule (leukocyte common antigen, LCA) in CD45.1-expressing strains such as SJL/J, DA, STS/A, and RIII. CD45.1 is expressed by all leukocytes in these strains.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested with mouse splenocytes (≤1.0 µg/test in 100 µL volume)

Brilliant Ultra Violet™ 661 (BUV661) emits at 660 nm and is optimized for ultraviolet (355 nm) laser-equipped cytometers.

When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.

Light Sensitivity: Protect from light to avoid photo-induced oxidation.
Fixation: Samples can be stored in IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.

Excitation: 350 nm
Emission: 660 nm
Laser: Ultraviolet laser

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells, comprising ~10% of surface proteins in lymphocytes. It regulates Src-family kinases, playing a key role in lymphocyte development and antigen signaling. Multiple isoforms exist due to alternative splicing, differing in extracellular domains. CD45 also modulates integrin-mediated signaling and is involved in apoptosis-related DNA fragmentation. CD45RA is one isoform with restricted lymphoid cell subtype expression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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