Thermo Fisher Scientific CD45.1 Monoclonal Antibody (A20), Brilliant Ultra Violet 661, eBioscience
마우스 CD45.1 항원 검출용 A20 단클론 항체로, Brilliant Ultra Violet 661 형광체가 결합된 제품입니다. Flow cytometry에 최적화되어 있으며, CD45.1 발현 마우스 백혈구 분석에 사용됩니다. 4°C 보관, 빛 차단 필요.
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD45.1 Monoclonal Antibody (A20), Brilliant Ultra Violet 661, eBioscience
Thermo Fisher Scientific CD45.1 Monoclonal Antibody (A20), Brilliant Ultra Violet 661, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 1.0 µg/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), Brilliant Ultra Violet™ 661, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | A20 |
| Conjugate | Brilliant Ultra Violet™ 661 |
| Excitation / Emission Max | 349 / 659 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2929054 |
Additional Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 532
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395
- Brilliant UV 563
- Brilliant UV 737
- Brilliant UV 805
- Brilliant Violet 421
- Brilliant Violet 480
- Brilliant Violet 605
- Brilliant Violet 650
- Brilliant Violet 711
- Brilliant Violet 786
- eFluor 450
- eFluor 506
- FITC
- NovaFluor Blue 510
- NovaFluor Blue 610-30S
- NovaFluor Blue 660-40S
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Request custom conjugation available
Product Specific Information
The A20 monoclonal antibody reacts with the mouse CD45 molecule (leukocyte common antigen, LCA) in CD45.1-expressing strains such as SJL/J, DA, STS/A, and RIII. CD45.1 is expressed by all leukocytes in these strains.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Tested with mouse splenocytes (≤1.0 µg/test in 100 µL volume)
Brilliant Ultra Violet™ 661 (BUV661) emits at 660 nm and is optimized for ultraviolet (355 nm) laser-equipped cytometers.
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.
Light Sensitivity: Protect from light to avoid photo-induced oxidation.
Fixation: Samples can be stored in IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.
Excitation: 350 nm
Emission: 660 nm
Laser: Ultraviolet laser
BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.
Target Information
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells, comprising ~10% of surface proteins in lymphocytes. It regulates Src-family kinases, playing a key role in lymphocyte development and antigen signaling. Multiple isoforms exist due to alternative splicing, differing in extracellular domains. CD45 also modulates integrin-mediated signaling and is involved in apoptosis-related DNA fragmentation. CD45RA is one isoform with restricted lymphoid cell subtype expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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