Thermo Fisher Scientific CD45.2 Monoclonal Antibody (104), Brilliant Ultra Violet 661, eBioscience

Thermo Fisher Scientific CD45.2 Monoclonal Antibody (Clone 104), Brilliant Ultra Violet 661

Applications

• Flow Cytometry (Flow): Tested at ≤ 1.0 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 496 / 563 / 615 / 737 / 805
• Brilliant Violet 421 / 605 / 650 / 711 / 786
• eFluor 450 / 506 / FITC
• NovaFluor Blue 510 / 555 / 585 / 610-70S / 660-120S
• PE / PE-Cyanine7 / PE-eFluor 610 / PerCP-Cyanine5.5
• Super Bright 436 / 600 / 645 / 780
• Request custom conjugation available

Product Specific Information

The 104 monoclonal antibody reacts with mouse CD45 molecule (leukocyte common antigen, LCA) in CD45.2-expressing strains such as C57BL/6, BALB/c, DBA, C3H/He, CBA, 129, A, and AKR. CD45.2 is expressed by all leukocytes in these strains.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Mouse splenocytes, ≤ 1.0 µg/test (100 µL final volume)
  • Cell number range: 10⁵–10⁸ cells/test
  • Antibody titration recommended for optimal performance

Brilliant Ultra Violet™ 661 (BUV661) emits at 660 nm and is optimized for cytometers equipped with a UV (355 nm) laser.

When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

Light Sensitivity: Protect vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in the dark.

Excitation: 350 nm
Emission: 660 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD45.2 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells, comprising ~10% of lymphocyte surface proteins. It regulates Src-family kinases, lymphocyte development, and antigen signaling. Multiple isoforms exist due to alternative splicing, differing in extracellular domains and membrane localization. CD45.2 also modulates integrin-mediated signaling and is involved in apoptosis-related DNA fragmentation. CD45RA is an isoform with restricted lymphoid subtype expression.

For Research Use Only. Not for use in diagnostic procedures.

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