Thermo Fisher Scientific CD45.2 Monoclonal Antibody (104), Brilliant Ultra Violet 661, eBioscience
Mouse CD45.2 단백질을 인식하는 104 클론 단일클론 항체로, Brilliant Ultra Violet 661 형광체로 결합되어 있습니다. Flow cytometry 분석에 적합하며, 모든 백혈구에서 발현되는 CD45.2 검출용으로 사용됩니다. 4°C 암소 보관, 비동결 권장.
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Thermo Fisher Scientific CD45.2 Monoclonal Antibody (Clone 104), Brilliant Ultra Violet 661
Applications
- Flow Cytometry (Flow): Tested at ≤ 1.0 µg/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), Brilliant Ultra Violet™ 661, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 104 |
| Conjugate | Brilliant Ultra Violet™ 661 |
| Excitation / Emission Max | 349 / 659 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2929056 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 532
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 496 / 563 / 615 / 737 / 805
- Brilliant Violet 421 / 605 / 650 / 711 / 786
- eFluor 450 / 506 / FITC
- NovaFluor Blue 510 / 555 / 585 / 610-70S / 660-120S
- PE / PE-Cyanine7 / PE-eFluor 610 / PerCP-Cyanine5.5
- Super Bright 436 / 600 / 645 / 780
- Request custom conjugation available
Product Specific Information
The 104 monoclonal antibody reacts with mouse CD45 molecule (leukocyte common antigen, LCA) in CD45.2-expressing strains such as C57BL/6, BALB/c, DBA, C3H/He, CBA, 129, A, and AKR. CD45.2 is expressed by all leukocytes in these strains.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Mouse splenocytes, ≤ 1.0 µg/test (100 µL final volume)
- Cell number range: 10⁵–10⁸ cells/test
- Antibody titration recommended for optimal performance
Brilliant Ultra Violet™ 661 (BUV661) emits at 660 nm and is optimized for cytometers equipped with a UV (355 nm) laser.
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.
Light Sensitivity: Protect vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in the dark.
Excitation: 350 nm
Emission: 660 nm
Laser: Ultraviolet Laser
BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.
Target Information
CD45.2 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells, comprising ~10% of lymphocyte surface proteins. It regulates Src-family kinases, lymphocyte development, and antigen signaling. Multiple isoforms exist due to alternative splicing, differing in extracellular domains and membrane localization. CD45.2 also modulates integrin-mediated signaling and is involved in apoptosis-related DNA fragmentation. CD45RA is an isoform with restricted lymphoid subtype expression.
For Research Use Only. Not for use in diagnostic procedures.
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