Thermo Fisher Scientific EAPP Polyclonal Antibody
Human EAPP 단백질을 인식하는 Rabbit Polyclonal Antibody로, IHC(P) 및 ICC/IF에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, PBS/glycerol buffer에 보존. 세포주기 조절 및 apoptosis 관련 연구에 적합.
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Applications
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:200–1:500
Immunocytochemistry (ICC/IF)
- Tested Dilution: 0.25–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human EAPP. Recombinant protein control fragment (Product #RP-89046). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.3 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2640862 |
Product Specific Information
Immunogen sequence:
VLLHGTPDQK RKLIRECLTG ESESSSEDEF EKEMEAELNS TMKTMEDKLS SLGTGSSSGN GKVATAPTRY YDDIYFDSDS EDEDRAVQVT KKKKKKQHKI PTNDELLYDP E
- Highest antigen sequence identity to the following orthologs:
- Mouse: 86%
- Rat: 86%
Target Information
This protein may play an important role in fine-tuning both major E2F1 activities: regulation of the cell cycle and induction of apoptosis. It promotes S-phase entry and inhibits p14(ARP) expression. The E2F family is crucial in cell cycle control and tumor suppressor protein interactions, and is targeted by transforming proteins of small DNA tumor viruses.
E2F proteins contain conserved domains including:
- DNA binding domain
- Dimerization domain (interacts with DP proteins)
- Transactivation domain (acidic amino acid-rich)
- Tumor suppressor protein association domain
The C-terminal of EAPP binds the N-terminal of E2F1 and interacts with E2F2 and E2F3, but not E2F4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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