Thermo Fisher Scientific EpCAM Polyclonal Antibody
EpCAM 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC/IF 등에 사용 가능. Human, Mouse, Rat에 반응하며 항원 친화 크로마토그래피로 정제됨. EpCAM 관련 종양 연구 및 세포 접착 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | View 3 publications |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:40–1:160 | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1–5 µg/mL | View publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human CD326 / EpCAM / Epithelial Specific Antigen |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.9 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 1% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10984102 |
Product Specific Information
This antibody is predicted to react with cow, pig, and rhesus monkey based on sequence homology.
Target Information
Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein expressed in epithelial tissues with a molecular weight of approximately 40 kDa. It functions as a calcium-independent cell adhesion molecule influencing cell cycle, proliferation, and metabolism through regulation of proto-oncogene c-myc and cell cycle genes cyclin A and E.
Ep-CAM-mediated adhesions negatively regulate cadherin-mediated adhesions, affecting epithelial differentiation and growth. Overexpression of Ep-CAM is associated with increased epithelial proliferation and is commonly observed in human carcinomas.
Ep-CAM serves as a marker for epithelial lineage tumors and is expressed on the basolateral surface of most simple epithelia and many carcinoma types. It can also help distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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