Thermo Fisher Scientific CD27 Monoclonal Antibody (O323), Super Bright 702, eBioscience

Thermo Fisher Scientific CD27 Monoclonal Antibody (O323), Super Bright 702, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.25 µg)/test
• View 6 publications

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV / Violet / NovaFluor / PE / FITC / Super Bright variants
• Request custom conjugation

Product Specific Information

The O323 monoclonal antibody reacts with human CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by a subset of thymocytes and virtually all mature T cells and is upregulated upon T-cell stimulation. CD27 binds to CD70, playing an important role in T cell–B cell interaction.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and tested on normal human peripheral blood cells
  • Use at 5 µL (0.25 µg) per test
  • A test = staining a cell sample in 100 µL final volume
  • Recommended cell count: 10⁵–10⁸ cells/test

Super Bright 702 is a tandem dye excited with a violet laser (405 nm) and emits at 702 nm. Recommended filter: 710/50 bandpass.
Use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.
Light-sensitive: Protect vial and stained samples from light.

Fixation:
Samples can be stored up to 3 days in the dark at 4°C using:

• IC Fixation Buffer (Product # 00-8222)
• 1-step Fix/Lyse Solution (Product # 00-5333)

Excitation: 405 nm; Emission: 702 nm; Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD27 is a 50 kDa member of the TNF receptor superfamily involved in regulation of cell proliferation and death. Expression of CD27 and its ligand CD70 is mainly confined to lymphocytes. CD27 forms disulfide-linked homodimers on mature thymocytes, peripheral blood T cells, and a subset of B cells. Activation via TCR-CD3 complex increases CD27 expression and release of soluble CD27 (sCD27), a prognostic marker in B cell malignancies.
CD27-binding protein (SIVA) is a proapoptotic protein involved in CD27-mediated apoptosis.
Diseases associated with CD27 dysfunction include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.

For Research Use Only. Not for use in diagnostic procedures or resale without express authorization.

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