Thermo Fisher Scientific MYO1A Polyclonal Antibody

Applications

• Immunohistochemistry (Paraffin) (IHC (P)): 1:200–1:500

Product Specifications

Product Specific Information

Immunogen sequence:
FSLHLSEMSSV GSKGDFLLVS EHVIELLTKM YRAVLDATQR QLTVTVTEKF SVRFKENSVA VKVVQGPAGG DNSKLRY

Highest antigen sequence identity to the following orthologs:

• Mouse: 82%
• Rat: 78%

Target Information

The MYO1A protein belongs to the myosin superfamily, which are molecular motors that interact with actin filaments and use ATP hydrolysis to generate mechanical force. Each myosin contains a conserved N-terminal motor domain with ATP- and actin-binding sites, followed by a neck region with IQ motifs that bind calmodulin or other EF-hand calcium-binding proteins. The C-terminus has a distinct tail domain responsible for dimerization, membrane binding, and targeting the myosin to specific subcellular locations.

In kidney epithelial cell line LLC-PK1-CL4 (CL4), MYO1A forms a brush border on the apical surface. The brush border population of MYO1A turns over rapidly, with its head and tail domains transiently interacting with core actin and plasma membrane, respectively. A rapidly exchanging pool of MYO1A surrounds a static actin core bundle.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.