Thermo Fisher Scientific Gemin 2 Recombinant Rabbit Monoclonal Antibody (ARC2419)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

ARC2419

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Gemin 2 (O14893) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Gemin 2,uniProtId:O14893-1,ncbiNodeId:9606,antigenRange:1-100,antigenLength:280,antigenImageFileName:MA5-37886_Gemin_2_O14893-1_Rabbit.svg,antigenImageFileNamePDP:MA5-37886_Gemin_2_O14893-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2897806

Product Specific Information

Positive Samples: U2OS, HeLa, MCF7, Mouse testis, Mouse thymus, Rat testis, Rat pancreas

Immunogen sequence: MRRAELAGLK TMAWVPAESA VEELMPRLLP VEPCDLTEGF DPSVPPRTPQ EYLRRVQIEA AQCPDVVVAQ IDPKKLKRKQ SVNISLSGCQ PAPEGYSPTL

Target Information

SIP1 is one of the proteins found in the SMN complex, which consists of the survival of motor neuron (SMN) protein and several gemin proteins. The SMN complex is localized to a subnuclear compartment called gems (gemini of coiled bodies) and is required for assembly of spliceosomal snRNPs and for pre-mRNA splicing. SIP1 interacts directly with the SMN and it is required for formation of the SMN complex. A knockout mouse targeting the mouse homolog of this gene exhibited disrupted snRNP assembly and motor neuron degeneration. However, knockdown of the SIP1 mRNA in motor neurons showed normal motor axons while that of SMN mRNA did show abnormal motor axon outgrowth, indicating that SIP1 may have additional roles outside of the SMN complex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.