Thermo Fisher Scientific GARP Monoclonal Antibody (G14D9), eFluor 660, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.5 µg)/test
• Publications: View 2 publications

Product Specifications

Available Formats

• FITC
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

Description:
The G14D9 monoclonal antibody reacts with human Glycoprotein A Repetitions Predominant (GARP, also known as LRRC32 or Garpin). GARP is an approximately 80 kDa glycoprotein expressed on the cell surface. Expression has been observed in placenta, lung, kidney, heart, ovary, liver, skeletal muscle, and pancreas, as well as on megakaryocytes, platelets, and activated regulatory T (Treg) cells. Expression of GARP on activated Treg cells is necessary for their suppressive function, possibly through its role as a receptor for LAP/TGF beta.

Applications Reported:
This G14D9 antibody is reported for use in flow cytometric analysis.

Applications Tested:
Pre-titrated and tested for flow cytometric analysis of stimulated normal human peripheral blood cells. Recommended use at 5 µL (0.5 µg) per test in a final volume of 100 µL. Cell number should be determined empirically (10⁵–10⁸ cells/test).

Fluorochrome Information:
eFluor® 660 is a replacement for Alexa Fluor® 647, emits at 659 nm, and is excited with the red laser (633 nm). Ensure instrument compatibility.

• Excitation: 633–647 nm
• Emission: 668 nm
• Laser: Red
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

GARP (Leucine Rich Repeat Containing 32) is a type I membrane protein with 22 leucine-rich repeats and a short intracytoplasmic region. It was identified through gene expression profiling of Treg and Th cells following TCR stimulation. Since GARP is expressed in CD4⁺, CD25⁺, FoxP3⁺ cells (Tregs), it serves as a surface marker candidate for Treg identification.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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