Thermo Fisher Scientific Human PLK1, His Tag Recombinant Protein
인간 PLK1 단백질을 His-tag 형태로 발현한 재조합 단백질로, 세린/트레오닌 키나아제 활성을 연구하는데 적합합니다. 인세크트 세포에서 발현되며 고순도(85%)로 정제되었습니다. 세포 분열 및 암 발생 관련 연구용으로 사용됩니다.
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Thermo Fisher Scientific Human PLK1, His Tag Recombinant Protein
Applications
- Kinase Assay (KA)
Tested Dilution
- Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | Insect cells |
| Amino Acid Sequence | Full length |
| Tag | His-tag |
| Molecular Weight | 73.3 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | 85% by SDS-PAGE |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Purified |
| Storage Buffer | 20mM HEPES, pH 7.5, with 0.05% Triton X-100, 50% glycerol, 5mM DTT, 0.5mM EDTA, 150mM NaCl, 0.5mM EGTA |
| Contains | No preservative |
| Storage Conditions | -80°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Dry ice |
Product Specific Information
- For maximum recovery, please spin prior to use.
- Aliquots of the 5 µg, 10 µg, and 20 µg sizes are not recommended; use in original packaging until exhausted.
- For larger sizes, reduce freeze/thaw cycles by preparing aliquots (not below 20 µL).
- Never store kinase diluted.
- Properly stored at -80°C, this product is guaranteed for 6 months from date of purchase.
- Protein Form: Full Length
Target Information
PLK1 is a serine/threonine kinase involved in cell mitosis and serves as a marker of cell proliferation. It plays a critical role in carcinogenesis. Polo-like kinase proteins act by binding and phosphorylating proteins that are already phosphorylated on specific motifs recognized by the POLO box domains.
PLK1 phosphorylates multiple substrates including BORA, BUB1B/BUBR1, CCNB1, CDC25C, CEP55, ECT2, ERCC6L, FBXO5/EMI1, FOXM1, KIF20A/MKLP2, CENPU, NEDD1, NINL, NPM1, NUDC, PKMYT1/MYT1, KIZ, PPP1R12A/MYPT1, PRC1, RACGAP1/CYK4, SGOL1, STAG2/SA2, TEX14, TOPORS, TP73, TPT1, and WEE1.
It plays a key role in centrosome function and bipolar spindle assembly by phosphorylating KIZ, NEDD1, and NINL.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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