Thermo Fisher Scientific Human PLK1, His Tag Recombinant Protein

Thermo Fisher Scientific Human PLK1, His Tag Recombinant Protein

Applications

• Kinase Assay (KA)

Tested Dilution

• Assay-dependent

Product Specifications

Product Specific Information

• For maximum recovery, please spin prior to use.
• Aliquots of the 5 µg, 10 µg, and 20 µg sizes are not recommended; use in original packaging until exhausted.
• For larger sizes, reduce freeze/thaw cycles by preparing aliquots (not below 20 µL).
• Never store kinase diluted.
• Properly stored at -80°C, this product is guaranteed for 6 months from date of purchase.
• Protein Form: Full Length

Target Information

PLK1 is a serine/threonine kinase involved in cell mitosis and serves as a marker of cell proliferation. It plays a critical role in carcinogenesis. Polo-like kinase proteins act by binding and phosphorylating proteins that are already phosphorylated on specific motifs recognized by the POLO box domains.
PLK1 phosphorylates multiple substrates including BORA, BUB1B/BUBR1, CCNB1, CDC25C, CEP55, ECT2, ERCC6L, FBXO5/EMI1, FOXM1, KIF20A/MKLP2, CENPU, NEDD1, NINL, NPM1, NUDC, PKMYT1/MYT1, KIZ, PPP1R12A/MYPT1, PRC1, RACGAP1/CYK4, SGOL1, STAG2/SA2, TEX14, TOPORS, TP73, TPT1, and WEE1.
It plays a key role in centrosome function and bipolar spindle assembly by phosphorylating KIZ, NEDD1, and NINL.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.