Thermo Fisher Scientific L-Plastin Polyclonal Antibody
Thermo Fisher Scientific의 L-Plastin Polyclonal Antibody는 인간 및 마우스 시료에 반응하며 Western blot, IHC, ICC에 적합합니다. 항원 친화 크로마토그래피로 정제된 고순도 항체로, 다양한 세포주에서 양성 대조로 사용 가능합니다. 연구용 전용 제품입니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human L-Plastin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.28 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792360 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (98%), Rat (97%), Zebrafish (86%), Xenopus laevis (88%), Chicken (92%), Rhesus Monkey (99%), Bovine (97%).
- Positive Control: A549, H1299, HCT-116, Raji, K562, THP-1, HL-60, NIH-3T3, JC, BCL-1.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
L-plastin is a leukocyte-specific actin bundling protein from the fimbrin family. It contains two actin-binding domains and two EF-hand type calcium-binding domains. The protein is phosphorylated on a serine residue in response to inflammatory stimuli and may regulate integrin-mediated adhesion in leukocytes.
L-plastin is expressed in most human cancer cell lines derived from solid tumors and is upregulated by testosterone in androgen receptor-positive prostate and breast cancer cells through androgen receptor binding elements located upstream of the gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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