Thermo Fisher Scientific UNG Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:1,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:50–1:200

Product Specifications

Product Specific Information

• Purity is >95% by SDS-PAGE.

Target Information

The human UNG gene encodes both nuclear (UNG2) and mitochondrial (UNG1) forms of uracil-DNA glycosylase. These forms are generated by alternative splicing and the use of two differentially regulated promoters, PA and PB.
The cDNAs for UNG1 and UNG2 are of similar size (2061 and 2058 bp, respectively), and the corresponding mRNAs are not resolved as two species in gel electrophoresis.
Nuclear UNG2 differs from mitochondrial UNG1 in 44 amino acids of the N-terminal sequence, which is not necessary for catalytic activity.

A major role of the UNG gene products is to repair mutagenic U:G mispairs caused by cytosine deamination. For example, UNG2 removes misincorporated dUMP residues.
The level and expression pattern of UNG1 and UNG2 differ between cell and tissue types. Additionally, UNG expression is cell cycle regulated, and its expression and activity are generally higher in proliferating than in nonproliferating tissues and cells.
UNG1 is a 304 amino acid protein, and UNG2 is a 313 amino acid protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.