Thermo Fisher Scientific HIC1 Polyclonal Antibody

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:20–1:50

Immunocytochemistry (ICC/IF)

• Tested Dilution: 0.25–2 µg/mL

Product Specifications

Product Specific Information

Immunogen sequence:
ELLAQTTHFL HDPKVALESL YPLAKFTAEL GLSPDKAAEV LSQGAHLAAG PDGRTIDRFS PT

Highest antigen sequence identity to the following orthologs:

• Mouse: 98%
• Rat: 97%

Target Information

Hypermethylated in cancer (HIC-1) was originally identified as a target of p53-induced gene expression.
HIC-1 is deleted in the genetic disorder Miller-Dieker syndrome (MDS), and its expression is frequently suppressed in leukemia and various cancers due to hypermethylation of specific DNA regions and transcriptional silencing.
Studies indicate that HIC-1 acts as a putative tumor suppressor protein mediating transcriptional repression.
HIC-1 is ubiquitously expressed in adult tissues and contains five zinc fingers and an N-terminal POZ/BTB domain.
This domain interacts with transcriptional repression complexes (SMRT/N-CoR-mSin3A HDAC), similar to BCL-6 and PLZF proteins.
However, HIC-1 does not directly interact with HDAC complex components, suggesting its repression mechanism is independent of the POZ/BTB domain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.