Thermo Fisher Scientific GLI1 Monoclonal Antibody (OTI1B4), TrueMAB
Human GLI1 단백질(aa 1-410)에 특이적인 Mouse IgG1 단클론 항체. Western blot에서 1:2,000 희석으로 검증됨. 동결건조 형태로 제공되며, 재구성 후 1 mg/mL 농도 유지. 줄기세포 증식 조절 관련 GLI1 단백질 연구용.
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Thermo Fisher Scientific GLI1 Monoclonal Antibody (OTI1B4), TrueMAB
Applications
- Western Blot (WB): Tested dilution 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1B4 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–410 of human GLI1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
- For conjugation experiments, perform an additional round of desalting using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
GLI1 encodes a member of the Kruppel family of zinc finger proteins. This transcription factor is activated by the Sonic Hedgehog signal transduction cascade and regulates stem cell proliferation. Its activity and nuclear localization are negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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