Thermo Fisher Scientific p27Kip1 (Mitotic Inhibitor/Suppressor Protein) Monoclonal Antibody (KIP1/769)
세포 주기 조절 단백질 p27Kip1을 검출하는 Thermo Fisher Scientific의 단클론 항체입니다. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합하며, 인간 및 설치류 등 여러 종에 반응합니다. 고순도 Protein A/G 정제, 액상 형태로 제공됩니다.
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Thermo Fisher Scientific p27Kip1 (Mitotic Inhibitor/Suppressor Protein) Monoclonal Antibody (KIP1/769)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 0.25–0.5 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | KIP1/769 |
| Immunogen | Recombinant full-length human CDKN1B protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
- CF 488 conjugate available
- Custom conjugation upon request
Target Information
p27 Kip1 functions as a negative regulator of G1 phase progression and is proposed to mediate TGFβ-induced G1 arrest. It inhibits cyclin E- and cyclin A-CDK2 complexes and interacts with cyclin D-CDK4 complexes, affecting their assembly and activation depending on phosphorylation state. The protein (~27 kDa) undergoes CDK-dependent phosphorylation and ubiquitination by SCF complexes, enabling transition from quiescence to proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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