Thermo Fisher Scientific IRS1 Monoclonal Antibody (OTI3G10), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:150

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

IRS-1 is a major substrate of the insulin receptor and is phosphorylated in response to stimulation by insulin, IGF-1, and IL-4.
Phosphorylation occurs on serine, threonine, and tyrosine residues across tissues. Casein kinase II mediates part of the insulin-stimulated serine/threonine phosphorylation of IRS-1, with Thr 502 and Ser 99 identified as major phosphorylation sites.
IRS-1 includes three putative 14-3-3 binding sites (Ser 270, Ser 374, Ser 641). The motif around Ser 270 lies within the phosphotyrosine binding domain, mediating interaction with the insulin receptor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.