Thermo Fisher Scientific IRS1 Monoclonal Antibody (OTI3G10), TrueMAB
IRS1 단백질을 인식하는 Mouse monoclonal antibody로, Human, Mouse, Rat 시료에 반응. Western blot과 IHC(Paraffin)에 최적화. Lyophilized 형태로 제공되며, 재구성 및 conjugation 실험에 적합. 연구용으로만 사용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:150
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3G10 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–280 of human IRS1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
IRS-1 is a major substrate of the insulin receptor and is phosphorylated in response to stimulation by insulin, IGF-1, and IL-4.
Phosphorylation occurs on serine, threonine, and tyrosine residues across tissues. Casein kinase II mediates part of the insulin-stimulated serine/threonine phosphorylation of IRS-1, with Thr 502 and Ser 99 identified as major phosphorylation sites.
IRS-1 includes three putative 14-3-3 binding sites (Ser 270, Ser 374, Ser 641). The motif around Ser 270 lies within the phosphotyrosine binding domain, mediating interaction with the insulin receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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