Thermo Fisher Scientific eIF4E Polyclonal Antibody
eIF4E 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF에 사용 가능. 인간 및 생쥐 반응성 확인. 고순도 항원 친화 크로마토그래피 정제. 단기 4°C, 장기 -20°C 보관. 연구용으로만 사용.
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Thermo Fisher Scientific eIF4E Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human eIF4E (exact sequence proprietary) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.52 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2735758 |
Product Specific Information
- Positive Control: 293T, 293T cytoplasm fraction extract, mouse colon
- Predicted Reactivity: Rat (99%), Xenopus laevis (91%), Rabbit (99%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (99%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
eIF4E is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). EIF4E is a eukaryotic translation initiation factor that directs ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide existing as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide (EIF4A) with ATPase and RNA helicase activities, and a 220-kD polypeptide (EIF4G).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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