Thermo Fisher Scientific PKC alpha/beta/gamma Recombinant Rabbit Monoclonal Antibody (9O5J1)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:8,000

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Rat, Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

9O5J1

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 300-500 of human PKC alpha + beta + gamma. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PKC alpha/beta/gamma,uniProtId:P05129-1,ncbiNodeId:9606,antigenRange:300-500,antigenLength:697,antigenImageFileName:MA5-55525_PKC_alphabetagamma_P05129-1_Rabbit.svg,antigenImageFileNamePDP:MA5-55525_PKC_alphabetagamma_P05129-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:PKC alpha/beta/gamma,uniProtId:P05771-1,ncbiNodeId:9606,antigenRange:300-500,antigenLength:671,antigenImageFileName:MA5-55525_PKC_alphabetagamma_P05771-1_Rabbit.svg,antigenImageFileNamePDP:MA5-55525_PKC_alphabetagamma_P05771-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Immunogen Sequence: ELRQKFERAK ISQGTKVPEE KTTNTVSKFD NNGNRDRMKL TDFNFLMVLG KGSFGKVMLS ERKGTDELYA VKILKKDVVI QDDDVECTMV EKRVLALPGK PPFLTQLHSC FQTMDRLYFV MEYVNGGDLM YHIQQVGRFK EPHAVFYAAE IAIGLFFLQS KGIIYRDLKL DNVMLDSEGH IKIADFGMCK ENIWDGVTTK T

Target Information

Protein Kinase c alpha (PKC alpha) is an 77 kDa member of the conventional group (cPKCs: sensitive to calcium, diacylglycerol, phosphatidylserine and phorbol esters) of the PKC family of serine/ threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC alpha is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of a broad range of cellular functions including proliferation, differentiation, development, migration, cell cell adhesion, cell extracellular matrix adhesion, and solute transport. The activation loop threonine (threonine 497 in PKC alpha) of conventional PKCs is phosphorylated by phosphoinositide dependent kinase 1 (PDK1). This phosphorylation is necessary for the autophosphorylation of threonine 638 in the carboxy terminus of PKC alpha, a step that is critical for regulating the rate of PKC alpha dephosphorylation and inactivation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.