Thermo Fisher Scientific GAPVD1 Polyclonal Antibody

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:50–1:200

Immunocytochemistry (ICC/IF)

• Tested Dilution: 0.25–2 µg/mL

Product Specifications

Product Specific Information

Immunogen sequence:
AMTGSEEGDP RTKSSLGKFD KSCVAAFLDV VIGGRAVETP PLSSVNLLEG LSRTVVYITY SQLITLVNFM KSVMSGDQLR EDRMALDNLL ANLP

Antigen sequence identity:

• Mouse: 94%
• Rat: 94%

Target Information

GAPVD1 acts both as a GTPase-activating protein (GAP) and a guanine nucleotide exchange factor (GEF), participating in various processes such as endocytosis, insulin receptor internalization, and LC2A4/GLUT4 trafficking.
It acts as a GEF for the Ras-related protein RAB31 by exchanging bound GDP for free GTP, regulating LC2A4/GLUT4 trafficking.
In the absence of insulin, it maintains RAB31 in an active state and promotes a futile cycle between LC2A4/GLUT4 storage vesicles and early endosomes, retaining LC2A4/GLUT4 inside cells.
Upon insulin stimulation, it translocates to the plasma membrane, releasing LC2A4/GLUT4 from intracellular storage vesicles.
It is also involved in EGFR trafficking and degradation, possibly by promoting EGFR ubiquitination and subsequent proteasomal degradation.
It has GEF activity for Rab5 and GAP activity for Ras.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.